表皮生长因子对晶体上皮细胞增殖影响的研究

Effect of epidermal growth factor on proliferation of lens epithelial cells

目的评价表皮生长因子（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＥＧＦ）对晶体上皮细胞增殖的影响。方法在培养的牛和人晶体上皮细胞中添加ＥＧＦ，甲基噻唑基四唑（ｍｅｔｈｙｌｔｈｉａｚｏｌｙｌｔｅｔｒａｚｏｌｉｕｍ，ＭＴＴ）法测定细胞增殖情况以及抗ＥＧＦ受体（ＥＧＦｒｅｃｅｐｔｏｒ，ＥＧＦＲ）抗体对ＥＧＦ作用的影响。结果ＥＧＦ浓度为１０１～１０２μｇ／Ｌ对牛眼晶体上皮细胞增殖有促进作用，其中浓度为１０μｇ／Ｌ作用３天达到最大促增殖效果；ＥＧＦ浓度为１～１０２μｇ／Ｌ对人晶体上皮细胞增殖有促进作用，浓度为１０２μｇ／Ｌ具有最大促增殖效果。抗ＥＧＦＲ抗体浓度＞０５ｍｇ／Ｌ时特异性阻断ＥＧＦ对人晶体上皮细胞增殖的促进作用，浓度为３ｍｇ／Ｌ时阻断率达８１％。结论ＥＧＦ可促进晶体上皮细胞增殖，并提示人晶体上皮细胞表面存在特异性ＥＧＦＲ，ＥＧＦ与其受体结合后可促进细胞增殖，对白内障术后后囊混浊的发生发挥作用。

Objective To study the effect of epidermal growth factor (EGF) on lens epithelial cells (LECs). Methods Bovine LECs (bLECS) and human LECs (hLECs) in culture were treated with EGF. Then, MTT assay was used to examine the proliferation of the cells and the effect of antiEGF receptor (EGFR) antibody. Results BLECs showed marked proliferation depending on the concentration of EGF from 101102 g/L, and EGF at a concentration of 10 g/L on day 3 induced the highest increase of proliferation; EGF promoted proliferation of hLECs at the concentration of 1102 g/L and EGF of 102 g/L showed the highest promotive effect on proliferation. The antiEGFR antibody blocked the promotive effect of EGF on proliferation of hLECs when the concentration of this antibody was as low as 05 mg/L, and the blocking rate was 81% at the concentration of 3 mg/L. Conclusions EGF has a promotive effect on proliferation of LECs. It is suggested that there are specific EGFRs on the surface of hLECs. EGFs bound to their receptors stimulate proliferation of LECs and play an important role in occurrence and development of posterior capsular opacification after cataract surgery.