固定化脂肪酶催化酯交换制备母乳脂肪替代物

Production of human milk fat substitutes through immobilized lipase-catalyzed interesterification

椰子油、橄榄油和大豆油按质量比0.3∶0.2∶0.5比例混合,制备富含不饱和脂肪酸的混合脂肪酸。以Sn-2位富含棕榈酸的猪油和混合脂肪酸为底物,通过固定化脂肪酶Lipozyme TL IM的酯交换作用制备母乳脂肪替代物。经单因素试验确定最佳反应条件为:猪油与混合脂肪酸质量比1∶2.0,加酶量10%,反应温度65℃,反应时间8 h。反应产物甘油三酯总脂肪酸组成及Sn-2位脂肪酸组成与母乳脂肪相似。

Fatty acid blend rich in unsaturated fatty acids was prepared using oil blend（mass ratio of coconut oil to olive oil to soybean oil 0.3∶0.2∶0.5）.The human milk fat substitutes was produced through the enzymatic interesterification with immobilized lipase Lipozyme TL IM between lard in which palmitic acid at the Sn-2 position of its triacylglycerol backbone was abundant and the fatty acid blend.Single factor test was used to determine the optimal conditions as follows： mass ratio of lard to fatty acid blend 1∶2.0,enzyme dosage 10%,reaction temperature 65℃,reaction time 8 h.The total and Sn-2 position fatty acid composition of the product was similar to the human milk fat.