鱼腥草中有效化学成分对N-亚硝化反应的阻断作用

Effect of Chemical Components in Houttuynia cordata on the Blocking of N-Nitrosation Reaction

采用紫外-可见光谱法和气质联用色谱法对鱼腥草中有效化学成分(黄酮类化合物、抗坏血酸(VC)、多酚、多糖、甲基正壬酮)进行测定,并研究有效化学成分对N-亚硝化反应阻断能力的关系。结果表明:不同产地鱼腥草中各有效化学成分含量不存在显著性差异;不同干燥时间鱼腥草中,随着干燥时间的增加,除总糖含量逐渐增大外,其他有效化学成分均呈下降趋势。在干燥和新鲜鱼腥草中,VC、黄酮和总酚含量均与阻断率呈正相关的关系,甲基正壬酮和多糖含量与阻断率不存在相关性。结论:鱼腥草能阻断N-亚基化合物的内源性合成,且影响N-亚硝化反应的有效化学成分为黄酮、VC和多酚。

Objective： To explore the effect of chemical components in Houttuynia cordata on the blocking of N-nitrosation reaction. Methods： UV-visible spectrum and gas chromatography-mass spectrometry methods were used to determine the chemical components including flavonoids, vitamin C, polyphenols, polysaccharides and 2-undecenal in Houttuynia cordata for exploring their blocking capabilities to N-nitrosation reaction. Results： The contents of chemical components in Houttuynia cordata from different areas did not exhibit an obvious difference. Although the content of total sugar exhibited an increase trend, other chemical components exhibited a declined trend during the extension of drying time. The contents of vitamin C, flavonoids and total polyphenols were positively correlated with the blocking rate of N-nitrosation reaction. However, the contents of 2-undecenal and total sugar had no correlation with the blocking rate. Conclusion： Houttuynia cordata can prevent human endogenous nitro-compound synthesis. The major components for influencing N-nitrosation reaction were flavonoids, vitamin C and polyphenols.