两种羊肚菌胞内多糖体外抗氧化性

Antioxidant Activity of Intracellular Polysaccharides (IPS) from Morchella esculenta L.

用水提醇沉法提取北京羊肚菌(MB)和淮阴羊肚菌(MH)菌丝体胞内多糖(IPS1),经脱色及脱蛋白得纯化多糖(IPS2)。采用水杨酸法测定IPS1和IPS2对·OH的清除作用;邻苯三酚自氧化法测定IPS1和IPS2对O2·的抑制作用。结果表明:IPS1和IPS2均能明显地清除·OH和抑制O2·的能力,在1～5mg/mL质量浓度范围,抗氧化活性随质量浓度的增加而增加。MB和MH提取的IPS1和IPS2清除O2·能力差异不显著,MB和MH提取的IPS1清除·OH能力的差异也不显著,MH提取的IPS1和IPS2之间清除·OH能力差异显著,质量浓度为5mg/mL时,IPS1清除能力只有22.57%,而IPS2清除能力高达46.27%,表明MH的胞内多糖的清除·OH能力明显高于MB胞内多糖。

The crude intracellular polysaccharides （CIPSMB and CIPSMH） extracted from the mycelia of Morchella esculenta L. from Beijing （MB） and Huaiyin （MH） areas by water extraction and alcohol precipitation were subjected to decolorization and deproteinization to obtain purified CIPSMB and CIPSMH, denoted as PIPSMB and PIPSMH, respectively. The scavenging effects of the four samples on hydroxyl and superoxide anion free radicals were tested by salicylic acid method and pyrogallol autoxidation method, respectively. It was observed that all the four samples were able to obviously scavenge both free radicals, displaying a positive concentration-dependent response in a concentration range between 1 mg/mL and 5 mg/mL. No statistical significance in the ability to scavenge superoxide anion free radicals was found between CIPSMB and PIPSMB and between CIPSMH and PIPSMH. CIPSMB and CIPSMH had no significant difference in the ability to scavenge hydroxyl free radicals. However, there was a significant difference in the ability to scavenge hydroxyl free radicals observed between CIPSMH and PIPSMH; the scavenging rate of CIPSMH at a concentration of 5 mg/mL was only 22.57%, whereas that of PIPSMH was as high as 46.27%, indicating that purification greatly enhances the hydroxyl free radical scavenging capacity of intracellular polysaccharides from the mycelia of Morchella esculenta L. from Huaiyin area.