大黄鱼中复合腐败菌腐败能力的分析

Analysis of Spoilage Ability of Single and Mixed Spoilage Bacteria in Pseudosciaena crocea

通过分析接种腐败菌的大黄鱼无菌鱼块在贮藏中的感官、腐败代谢产物和腐败菌的变化,以腐败菌生长动力学参数和腐败代谢产物产量因子(YTVB-N/CFU和YTMA/CFU)为指标,探讨冷藏大黄鱼优势腐败菌(腐败希瓦氏菌、假单胞菌以及这两种菌的复合菌)的腐败能力。结果表明,接种腐败希瓦氏菌、假单胞菌和复合菌的无菌鱼块的货架期分别为168、174、168h,说明接种假单胞菌的货架期较长。腐败希瓦氏菌、假单胞菌和复合菌的YTVB-N/CFU基本一致,腐败希瓦氏菌的YTMA/CFU明显大于假单胞菌和复合菌,腐败希瓦氏菌的腐败能力较假单胞菌和复合菌强。假单胞菌对腐败希瓦氏菌的生长有一定的拮抗作用,但仅在较高数量时才有明显作用,腐败希瓦氏菌是有氧冷藏养殖大黄鱼的特定腐败菌。

Sensory quality, metabolic products of spoilage bacteria and change of spoilage bacteria were assayed on sterile Pseudosciaena crocea tissue blocks inoculated with spoilage bacteria, with YTVB-N/CFU and YTMA/CFU, the growth kinetic parameters of the spoilage bacteria and the yield factor of microbial metabolites, respectively, used as the index. Spoilage ability of two spoilage bacteria Shewanella putrefaciens and Pseudomonas spp., as well as the mixing of the two spoilage bacteria were analyzed on Pseudosciaena crocea stored aerobically at chilled condition. The results showed that shelf life was 168 h, 174 h, and 168 h, respectively, for sterile tissue blocks inoculated with Shewanella putrefaciens, Pseudomonas spp. and their mixture, while it was relatively longer with Pseudomonas spp. The YTVB-N/CFU for Shewanella putrefaciens, Pseudomonas spp. and mixed bacteria was basically similar, meanwhile YTMA/CFU for Shewanella putrefaciens was higher than for Pseudomonas spp. and mixed bacteria. The spoilage ability of Shewanella putrefaciens was more significant than Pseudomonas spp. and mixed bacteria. Pseudomonas spp. had inhibitory effect on he growth of Shewanella putrefaciens to some extent, however it was not significant until it reached high counts. Shewanella putrefaciens was specific spoilage organism in Pseudosciaena crocea stored at chilled temperature aerobically.