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Lactobacillus paracaseiW2合成苯乳酸培养条件的优化 被引量：6

Optimization of Culture Medium for Phenyllactic Acid Production by Lactobacillus paracasei W2
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摘要 对苯乳酸(PLA)高产菌株副干酪乳杆菌Lactobacillus paracaseiW2的培养条件进行优化,以提高PLA合成能力。采用Plackett-Burman(PB)试验设计法,筛选出3个主要影响因子:葡萄糖、吐温-80、苯基丙酮酸(PPA)。通过最陡爬坡法和响应面分析方法(RSM)确定葡萄糖质量浓度19.5g/L、吐温-804mL/L、苯基丙酮酸质量浓度3g/L。在此优化条件下,Lactobacillus paracaseiW2发酵合成苯乳酸的产量达到801mg/L,比优化前提高1.4倍。 The optimal medium for phenyllactic acid （PLA） production by Lactobacillus paracasei W2 was investigated in order to improve PLA yield. Three most significant components affecting PLA production were selected out of seven components using Plackett-Burman design, namely glucose, Tween-80 and phenylpyruvic acid. Their optimal concentrations were determined by steepest ascent method and response surface methodology （RSM） to be 19.5 g/L, 4 mL/L and 3 g/L, respectively. PLA production reached 801 mg/L under these conditions, 1.4-fold higher than before optimization.
作者 刘戈 王立梅
机构地区 吉林农业大学食品工程学院 常熟理工学院生物与食品工程系 苏州市食品生物技术重点实验室
出处 《食品科学》 EI CAS CSCD 北大核心 2010年第23期174-177,共4页 Food Science
基金 江苏省科学技术局(BE2008391) 常熟市科技局资助项目(CN200921)
关键词 LACTOBACILLUS paracaseiW2 苯乳酸 最陡爬坡法 PLACKETT-BURMAN设计 响应面分析 Lactobacillus paracasei W2 PLA steepest ascent method Plackett-Burman design response surface methodology
分类号 Q939.11 [生物学—微生物学]
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参考文献15

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共引文献52

同被引文献73

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引证文献6

二级引证文献44

食品科学
2010年 第23期

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