激素对蝉拟青霉深层液体发酵生长代谢的调控作用

Regulatory Effect of Hormones on the Growth and Metabolism of Paecilomyces cicadae in Submerged Fermentation

以生物量、腺苷产量、胞内多糖产量和虫草素产量为指标,考察不同添加量的细胞分裂素6-苄氨基嘌呤(6-BA)和保幼激素Ⅲ对蝉拟青霉深层液体发酵的调控作用。各实验组在整个发酵过程中的生长代谢节奏与对照组有一定的差异性,两种激素均具有提高蝉拟青霉生物量的作用,其中6-BA添加量为4mg/L时比对照组提前12h到达生物量最大值,并且其最大生物量较对照组最大生物量提高了28.96%。两种激素均可调节蝉拟青霉菌丝体中活性物质的代谢节奏,低剂量6-BA可显著提高胞内多糖产量(P<0.05);而保幼激素Ⅲ中、高两剂量组则在培养36h以后表现出了明显抑制多糖合成的作用(P<0.05)。虫草素和腺苷产量分析结果也表明激素可改变两种活性物质的代谢节奏。

The regulatory effects of separated added 6-benzylaminopurine and juvenile hormone III before the submerged fermentation of Paecilomyces cicadae on the mycelial growth, intracellular polysaccharide （IPS）, cordycepin and adenosine production were explored. The rhythm of mycelial growth and metabolism were different between the control group and the test groups during the whole fermentation process. Two hormones had an promotion effect on the maximum biomass of Paecilomyces cicadae, the biomass of Paecilomyces cicadae was increased by 28.96% and the fermentation time could shorten to 12 h when 6-benzylaminopurine was added at a concentration level of 4 mg/L. In addition, 6-benzylaminopurine and juvenile hormone III could regulate the metabolic rhythm of bioactive materials in Paecilomyces cicadae. The content of IPS was notably increased when 6-benzylaminopurine was added at the low concentration （2 mg/L） （P 〈 0.05）, and juvenile hormone III had an significant inhibitory effect at a concentration level of 4（middle） or 8 mg/L（high） after fermentation for 36 h （P 〈 0.05）. Furthermore, both kinds of hormones also could change the metabolic rhythm of cordycepin and adenosine based on their productivity during the fermentation.