富锗大麦苗体内外抗肿瘤作用

in vitro Anti-tumor Effect of Germanium-riched Barley Seedling

目的:研究富锗大麦苗的体内外抗肿瘤作用。方法:建立小鼠S-180肉瘤模型,富锗大麦苗连续灌胃12d,观察其对荷瘤小鼠瘤质量、胸腺指数和脾脏指数的影响;同时采用MTT法研究富锗大麦苗体外对小鼠S-180肉瘤细胞及小鼠SP-2骨髓瘤细胞增殖抑制作用。结果:富锗大麦苗高剂量组可明显抑制小鼠体内S-180肉瘤的生长(P<0.05),抑瘤率为68.52%,且明显提高小鼠的胸腺指数和脾脏指数(P<0.05);富锗大麦苗具有明显的抑制小鼠S-180肉瘤细胞及小鼠SP-2骨髓瘤细胞体外增殖的作用,且与富锗剂量呈正相关。结论:富锗大麦苗在体内和体外都有明显的抑制肿瘤增殖的作用。

Objective： To investigate the anti-tumor effect of Germanium-enriched barley seedling （GEBS） in vivo and in vitro. Methods： The S-180 model mice were administered with GEBS at different dosages by gavage for 12 consecutive days. The effect of GEBS on tumor weight, thymus index and spleen index of mice was explored. Meanwhile, the inhibition effect of GEBS on the proliferation of mouse sarcoma cell S-180 and myeloma cell SP-2 was also evaluated by MTT assay. Results： GEBS with high dosage could obviously suppress the growth of S-180 sarcoma in mice with an inhibition rate of 68.52% （P0.05） and increase the thymus index and spleen index （P0.05）. Moreover, GEBS could obviously inhibit the growth of mouse sarcoma cell S-180 and myeloma cell SP-2 in vitro in a positive dosage-dependent manner. Conclusion： GEBS can obviously inhibit the growth of tumor in vivo and in vitro.