复方紫草抗氧化剂的毒性初步评价

Preparation and Toxicity Evaluation of Compound Lithospermum Antioxidant

以乙醇提取法获得紫草提取物(Z)和甘草提取物(G),以碱提酸沉法获得槐米提取物(H),再辅以柠檬酸(N)和VE(E),按Z:G:H:N:E为10:5:5:2:1的比例复配成复方紫草抗氧化剂(FZK)。以FZK为受试物,通过小鼠经口急性毒性实验、小鼠骨髓细胞微核实验、小鼠骨髓细胞染色体畸变实验等毒理学实验对FZK的毒性进行了评价。结果表明:小鼠经口最大耐受剂量均大于16000mg/kgbw,FZK急性毒性分级为无毒级。剂量小于8000mg/kgbw条件下,FZK对小鼠嗜多染红细胞微核率无促提高作用,对小鼠骨髓细胞染色体无致畸作用。该食品添加剂不存在致突变性。

A compound Lithospermum antioxidant （FZK） was prepared using Lithospermum ethanol extract （Z）, licorice ethanol extract （G）, Sophora japonica extraction （H） from alkali extraction and acid precipitation, citric acid （N） and vitamin E （E） by the ratio of 10：5：5：2：1 （Z：G：H：N：E）. The toxicity of FZK was evaluated by acute toxicity test, micronucleus test of bone marrow cells in mice, and chromosomal aberration test of bone marrow cells. Results indicated that the maximum tolerance dose of oral administration in mice was 16000 mg/kg body weight. According to acute toxicity classification criteria, FZK is non-toxic. However, FZK didn＇t exhibit a role in improving micronucleus rate of polychromatic erythrocyte and the mutagenicity in mice at the dose of less than 8000 mg/kg body weight. ,