结核病患者血清小RNA表达谱型特征及其编码基因的染色体定位

Characteristics of the expression profiles of serum small RNA and of which the location of encoding genes in human chromosome in patients with tuberculosis

目的:了解结核病患者血清小RNA(sRNA)的表达特征及其编码基因的染色体分布,以探讨结核病发生、发展的调控机制。方法:应用离心柱层析法提取血清总RNA,采用Solexa深度测序技术对结核病患者和健康人群血清sRNA进行测序,对长度10～30nt的sRNA的表达进行分析;并将测得的所有sRNA序列与人类基因组进行比对,确定其在人类染色体上的定位。结果:对27例结核病患者和25例健康对照者血清sRNA进行了检测与分析,在10～30nt长度范围内,结核病患者不同长度血清sRNA的表达丰度呈近似正态分布,健康人群则呈明显的负偏态分布;结核病患者血清sRNA编码基因主要位于染色体chr17、chr8、chr22、chr9、chrun-g1000220的正义链和chr11、chr8的负义链上,健康人群血清RNA编码基因主要位于染色体chr17、chr22、chrun-g1000220的正义链和chr8的负义链上。结论:结核病患者有其特定的血清sRNA表达谱,其编码基因亦呈现特定的染色体分布。

Objective To explore the expression characteristics of serum small RNA （sRNA） and of which the distribution of encoding genes in human chromosome in patients with tuberculosis,and to investigate the regulatory mechanisms in the pathogenesis and development of tuberculosis. Methods The total RNA of serum from patients with tuberculosis （cases） and healthy people （controls）was abstracted by centrifugal column chromatography and the sRNA sequences were detected by Solexa deep sequencing technology. The expression of sRNA of 10～30 nt length was analyzed and position of all serum sRNA in human chromosome was located through comparison with human genome. Results The serum sRNA of 10～30 nt length from 27 cases and 25 controls was detected and analyzed. The abundance of kinds of sRNA displayed normal distribution in patients while significant negative skewness in controls. The corresponding encoding genes mainly located in sense strand of chr17,chr8,chr22,chr9,chrun-g1000220 and antisense strand of chr11,chr8 in patients,while sense strand of chr17,chr22,chrun-g1000220 and antisense strand of chr8 in controls. Conclusion There exist specific expression profiles of serum sRNA and of which exist the specific distribution of encoding genes in human chromosome in patients with tuberculosis.