腺病毒表达谷氨酸受体羧基肽段对大鼠脑缺血再灌注后Bcl-2磷酸化及与Bax结合的影响

Effects of adenovirus expressing glutamic acid receptor c-terminal peptide on the phosphorylation of Bcl-2 and the combination of Bcl-2 with Bax in rats with brain ischemia and reperfusion

目的:研究大鼠脑缺血再灌注后腺病毒表达谷氨酸受体羧基肽段对海马CA1区神经元的保护和Bcl-2磷酸化及与Bax结合的影响。方法:将SD大鼠随机分为假手术组、缺血再灌注对照组、表达病毒组、非表达病毒组及溶剂组。采用焦油紫染色、免疫沉淀和免疫印迹检测大鼠缺血再灌注后海马神经元损伤、脑组织Bcl-2的磷酸化及与Bax结合情况。结果:表达病毒组与缺血再灌注对照组、非表达病毒组及溶剂组相比海马CA1区神经元损伤明显减轻,脑组织中Bcl-2磷酸化明显降低,Bcl-2/Bax的结合明显升高(P<0.05)。结论:腺病毒表达谷氨酸受体羧基肽段可能通过降低脑组织中Bcl-2的磷酸化从而增加Bcl-2/Bax的结合,进而对海马CA1区神经元起保护作用。

Objective To study the effects of adenovirus expressing glutamic acid receptor c-terminal peptide paragraph on neuron,phosphorylation of Bcl-2 and the combination of Bcl-2 with Bax in hippocampal CA1 region of rats with brain ischemia and reperfusion. Methods SD rats were randomly divided into 5 groups：sham-operation group （group A）,cerebral ischemia and reperfusion group （group B）,cerebral ischemia and reperfusion＋ expressing virus treatment group （group C）,cerebral ischemia and reperfusion ＋ no-expressing virus treatment group （group D） and reperfusion＋solvent （group E）. Brain injury in hippocampus of rats and change of phosphorylation of Bcl-2 and its combination with Bax was detected by Cresly Violet-strained or Immunoblot assay. Results As compared with those in group B,D,E,the injury in hippocampus CA1 region and phosphorylation of Bcl-2 were significantly decreased,while the combination of Bcl-2 with Bax was increased in group C （P 0.05）. Conclusions Adenovirus expressing glutamic acid receptor c-terminal peptide can protect the neurons in hippocampus from ischemia by decreasing phosphorylation of Bcl-2 and increasing the combination of Bcl-2 with Bax.