摘要
研究了聚乙二醇4000-硫酸铵(PEG4000/(NH4)2SO4)双水相体系中,PEG浓度、硫酸铵浓度及pH对从商品纤维素酶样品中萃取纤维素酶的影响。结果表明:双水相体系的质量组成为(NH4)2SO4 8%,PEG4000 26%,pH4.8时,分配系数K为5.21,萃取率为79.4%。
The effects of concentration of polyethylene glycol,ammonium sulfate and pH on extraction of cellulase from industrial cellulase in aqueous two-phase system of PEG-4000 and ammonium sulfate were studied.The results show that： The conditions of aqueous two-phase systems extraction are as folows： concentration of（NH4）2SO4 is 8%,concentration of PEG4000 26%,pH4.8,The partition coefficient K and the recovery rate were 5.21 and 79.4% respectively.
出处
《食品科技》
CAS
北大核心
2010年第12期191-193,共3页
Food Science and Technology
基金
陕西省科技攻关项目(2007JK01-13-2)
咸阳市科技攻关项目资助(K0311-2)
关键词
纤维素酶
双水相
分配系数
cellulase
aqueous two-phase systems
partition coefficient
