摘要
为了建立一种快速测定乳蛋白水解液中多肽含量的方法,采用浓度为10%三氯乙酸沉淀蛋白水解液中的大分子蛋白质,经离心过滤后,在上清液中加入双缩脲试剂,于310 nm下测定其OD值,继而在谷胱甘肽三肽标准曲线上查出样品中的多肽含量的方法。
In order to establish a rapid determination method of peptide content of milk protein hydrolysis,In the study macromolecule protein in protein hydrolysate was deposited by using 10% trichloroacetic acid.After centrifugalization and filtration,biuret reagent was added to the clear solution.The OD value was mensurated under 310 nm.Then the content of peptides of the sample was obtained through contrasted on the standard curve of GSH tetrapeptide.
出处
《食品科技》
CAS
北大核心
2010年第12期275-278,共4页
Food Science and Technology
关键词
双缩脲反应
三氯乙酸
多肽含量
biuret reaction
trichloroacetic acid
content of peptides
