性病门诊患者HPV-DNA荧光定量检测结果分析

Detection of Human Papilloma Virus DNA by fluorescence quantitave PCR in outpatients with venereal disease

目的分析性病门诊患者HPV-DNA荧光定量检测结果。方法采用荧光定量聚合酶链反应技术（FQ-PCR）对102例性病门诊患者进行HPV-DNA分型检测。结果 102例患者中HPV6/11型阳性率为27.66%,HPV16/18型阳性率为9.09%,分型结果以HPV6/11型为主,而HPV6/11型感染主要集中在20~40岁,其中20~30岁阳性率最高;HPV16/18型感染年龄均在40岁以上,其中以41~50岁较高,男、女HPV阳性率分别为7.89%和23.44%,差异有显著性。结论 HPV6/11型是HPV感染的主要型别,荧光定量聚合酶链反应（FQ-PCR）可作为尖锐湿疣诊断的指标,应加强对性病门诊患者的HPV检测。

Objective To analyze the result of human papilloma virus（HPV） DNA detection by fluorescence quantitative PCR in outpatients with venereal diseases.Methods The level of human papilloma virus DNA in 102 outpatients with venereal disease was measured by fluorescent quantitative polymerase chain reaction.Results In 102 outpatients,the positive rate of HPV6/11 was 27.66% while 9.09% was HPV16/18.The HPV6/11 infection was the main type,which was centradized between 20 to 40 years old,and mostly occurred from 20 to 30 years old.The infection of HPV16/18 was centradized over 40 years old,which was mostly occurred between 41 to 50 years old.The positive rate of HPV was 7.89% in male outpatients while was 23.44% in female outpatients,there were significant differences in both groups.Conclusion The HPV6/11 is the main type of infection.FQ-PCR can be taken as an index in CA diagnosis,It should be strengthened to detect HPV infection of outpatients with venereal disease.