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miRNA-21/pG-super真核表达载体构建及其在肺泡巨噬细胞RAW264.7中的表达

Construction of eukaryotic expression vector miRNA-21/pG-super and its expression in alveolar macrophage RAW264.7 cells
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摘要 为了研究miRNA-21在免疫细胞中的调控作用,试验构建了miRNA-21过表达载体,并将其成功转染到RAW264.7细胞中。结果表明:miRNA-21在RAW264.7细胞中获得了大量表达,说明miRNA-21/pG-super真核表达载体构建成功。 To investigate regulation and control of miRNA-21 in immune cells,the overexpression vector of miRNA-21 was successfully transfected into RAW2647 cells.The results showed that miRNA-21 in RAW264.7 cells gotmuch expression;the eukaryotic expression vector miRNA-21/pG-super was successfully constructed.
作者 赵锡兰 汤建中 杨凤琴 徐文锦 赵婧 徐广贤
机构地区 宁夏医科大学检验学院
出处 《黑龙江畜牧兽医》 CAS 北大核心 2010年第12期20-21,24,共3页 Heilongjiang Animal Science And veterinary Medicine
基金 国家自然科学基金项目(30960275)
关键词 真核表达 MIRNA-21 肺泡巨噬细胞 eukaryotic expression miRNA-21 alveolar macrophage
分类号 Q812 [生物学—生物工程]
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黑龙江畜牧兽医
2010年 第12期

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