曲马多在急性中毒大鼠体内的分布

Distribution of Tramadol in Acute Poisoned Rats

目的建立一种快速、准确的气相色谱分析方法,研究曲马多在急性中毒大鼠体内的分布,为曲马多中毒案的法医学鉴定取材和结果评价提供参考。方法以1140mg/kg(5×LD50)剂量曲马多对大鼠灌胃染毒,运用气相色谱法检测其体液和组织中曲马多的浓度。结果血液、尿液中曲马多的检出限为0.1μg/mL,肝中的检出限为0.1μg/g。方法的日内及日间精密度分别在3.1%和5.5%以内,血液中曲马多的回收率在98%以上。曲马多在急性中毒大鼠体液和组织中的含量从高到低依此为心血、肝、外周血、尿液、玻璃体液、肾、肺、脾、心、大脑。结论所建方法能够满足体内毒物分析的要求,血液、尿液、肝、肺和肾均可作为体内曲马多分析的良好检材。

Objective To develop a rapid and accurate gas chromatography method and investigate the distribution of tramadol in acute poisoned rats for information of samples selection and results evaluation in forensic identification.Methods After an oral administration of tramadol at 1 140 mg/kg（5×LD50）,concentrations of tramadol in rats＇ biological fluids and tissues were determined by gas chromatography.Results The limit of detection of tramadol in blood and urine was 0.1 μg/mL and the limit of detection in liver was 0.1 μg/g.The intra-day precision and inter-day precision were within 3.1% and 5.5% respectively,and the recovery of tramadol in blood was more than 98%.The average levels of tramadol displayed in descending order of heart blood,liver,peripheral blood,urine,vitreous humor,kidney,lung,spleen,heart,brain respectively.Conclusion The established method could meet the requirements for toxicological analysis,and the results of the study suggest that blood,urine,liver,lung and kidney are suitable samples for forensic toxicological analysis in tramadol poisoning cases.