圩猪OB基因SNPs检测及其与产仔性能的关系

SNPs detection of OBgene and its association with reproduction in Wei pig

为探讨圩猪OB基因的单链核苷酸多态性及其与产仔性状之间的关系,本试验采用PCR-SSCP方法检测了70头圩猪OB基因第2、3外显子的遗传多态性。结果表明:1)圩猪群体OB基因外显子2上没有检测到突变,而外显子3所扩增的片段中存在3个突变位点(C3619G、G3620C和G3714T);2)在G3714T突变位点上检测出AA、AB和BB3种基因型,其频率分别为0.7000、0.2286和0.0714;群体中A的基因频率为0.8143,B的基因频率为0.1857;3)χ2检验表明,本研究群体OB基因外显子3的多态位点处于Hardy-Weinberg平衡状态;4)最小二乘分析表明,G3714T位点3种基因型在初产总产仔数、初产产活仔数及经产总产仔数、经产产活仔数性状上差异不显著(P>0.05),但呈现出BB>AB>AA的趋势。

To study the SNPs of the OB gene and its association with litter size in Wei pig,the polymorphisms of OB gene in Wei pig was analyzed by PCR-SSCP.The results showed that,1）there was no mutation in the second exon and three SNPs existed in the third exon（C3619G,G3620C,G3714T）.2）There are three genotypes（AA,AB and BB）in populations with allele frequencies of 0.700 0,0.228 6 and 0.071 4,respectively,where the frequency of the allele A is 0.814 3,and that of the allele B is 0.185 7.3）The test of Hardy-Weinberg equilibrium showed that genotypic frequencies of the third exon of OB gene were in equilibrium.4）The results of Least-square analysis in G3714 Tloci showed that,there were no significant（P0.05）difference among the three genotypes in total number born（TNB）of the first parity,number born alive（NBA）of the first parity,TNB of the latter parities and the NBA of the latter parities in Wei pigs,though the trend was BBABAA.