皖西白鹅繁殖周期中垂体FSHβ mRNA的表达规律

Expression of pituitary FSHβ mRNA during reproduction cycle in Wanxi white goose

为探索皖西白鹅繁殖周期中不同状态下垂体FSHβ mRNA的表达特性,选取开产前期(220日龄)、产蛋期、就巢起始期、就巢中后期和就巢后休产期的皖西白鹅母鹅各6只,采用荧光定量PCR方法分别测定了各时期垂体FSHβ mRNA的表达水平。结果显示,产蛋期母鹅垂体FSHβ mRNA表达水平为1862.9±350.5,极显著高于其他时期(P<0.01)。产蛋前、就巢起始、就巢中后期以及就巢后休产期母鹅垂体FSHβ mRNA表达水平分别为95.5±33.8、248.2±40.0、122.3±41.3和211.6±56.8,但各时期间的表达水平差异不显著(P>0.05)。由此可见,皖西白鹅垂体FSHβ mRNA表达水平与繁殖周期密切相关,且产蛋期该基因表达水平的大幅度下调与就巢行为起始和维持有关。

To investigate the expression of pituitary FSHβ mRNA at different stages during reproduction cycle in Wanxi white goose,the pituitary FSHβ mRNA level was tested by FQRT-PCR method in six female geese at stages of prelaying,laying,onset of incubation,middle or later period of incubation,and laying cessation after incubation,respectively.Results showed that,the level of pituitary FSHβ mRNA（1 862.9±350.5）in laying stage was higher（P 0.01）than which at any of other stages.The average levels of pituitary FSHβ mRNA were 95.5±33.8,248.2±40.0,122.3±41.3,and 211.6±56.8at pre-laying,onset of incubation,middle or later period of incubation,and laying cessation after incubation,respectively,but there is no significantly differ among them（P0.05）.Therefore,the level of pituitary FSHβ mRNA is highly associated with the reproduction cycle in Wanxi white goose,and a sharp decrease of pituitary FSHβ mRNA level in laying period associated with the onset and maintenance of incubation.