NO对石斛类原球茎内源激素水平及生物碱积累的影响

EFFECTS OF NITRIC OXIDE ON ENDOGENOUS HORMONES AND ALKALOIDS PRODUCTION OF Hybrid dendrobium PLBs

以杂交石斛类原球茎为材料,硝普钠(SNP)为NO的供体,研究了SNP处理对石斛类原球茎的内源激素水平及生物碱积累的影响。结果表明,SNP处理促进了类原球茎生物碱的积累,处理组类原球茎的生物碱含量比对照提高了41.89%,但类原球茎生长受到一定的抑制;SNP处理刺激类原球茎内源激素水平升高,同时苯丙氨酸解氨酶(PAL)活性显著增强,从而调控石斛类原球茎生物碱的积累;通径分析表明,多种内源激素通过相互作用调控石斛类原球茎生物碱的积累,水杨酸(SA)对生物碱含量的直接正效应排在首位。

The influences of nitric oxide（sodium nitroprusside used as the donor） on endogenous hormones and alkaloids production were investigated in suspension cultures of Hybrid dendrobium protocorm-like bodies（PLBs）.The results showed that sodium nitroprusside（SNP） treatment accelerated the accumulation of alkaloids and inhibited the PLBs growth.The alkaloids content of treated PLBs was 41.89% higher than that in untreated PLBs cultures.The level of endogenous hormones rised and the PAL activity of Hybrid dendrobium PLBs was significantly enhanced by SNP treatment.It was assumed that the changes were responsible for the regulation of the accumulation of alkaloids.Results of path analysis indicated that endogenous hormones regulated the accumulation of alkaloids by interactions.The direct effect of salicylic was the most important in suspension cultures of Hybrid dendrobium PLBs.