大孔吸附树脂分离夏枯草果穗总黄酮的研究

Separation and purification of flavonoids from Prunella Vulgaris Linn by macroporous adsorption resin

通过比较D-101、AB-8、X-5、DA-201、HZ-806和HZ-801 6种不同性质大孔吸附树脂对夏枯草果穗总黄酮的静态吸附和解吸附性能,筛选出适合夏枯草果穗总黄酮分离纯化的树脂,并对其进行动态吸附特性研究。静态吸附实验结果表明,弱极性AB-8大孔吸附树脂对夏枯草果穗总黄酮具有较好的吸附和解吸附效果。动态吸附实验得其最佳动态吸附分离工艺参数为：上柱原液pH为3.0-3.5,上柱液体积3BV/次,上柱速度6BV/h,上样液浓度为1.8mg/mL。洗脱液乙醇浓度70%,洗脱液流速6BV/h,洗脱液用量6BV/次。在此条件下,树脂使用1次时,总黄酮吸附率和回收率分别达92.36%和89.09%,总黄酮的纯度由41.58%提高到70.68%;树脂重复使用4次时,总黄酮的收率和纯度仍达80%和60%以上。

The characteristics of macroporous adsorption resins D-101,AB-8,X-5,DA-201,HZ-806,and HZ-801 on adsorption and desorption of total flavonoids from Prunella Vulgaris Linn were investigated.With the yield of flavonoids as index,the optimum process of separating and purifying flavonoids was screened by static and dynamic adsorption tests.The results of static adsorption test exhibited that AB-8 resin has higher absorption and desorption capacity.The optimum technology parameters of adsorption and desorption are pH 3.0-3.5,sample volume 3BV,sample concentration 1.8mg/mL,flow velocity 6BV/h.total flavonoids adsorbed on the column can be eluted by 70% alcohol at 6BV/h flow rate and at the volume of 6BV.Compared to the 41.58% of total flavonoids in crude extracts,the recovery and purity of total flavonoids were 92.36% and 89.09% respectively by the fresh resin.The recovery and purity of total flavonoids were over 80% and 60% respectively after resin used for four times.