HPLC-ELSD法同时测定饮料中四种高倍甜味剂的含量

Determination of high sweetener in beverage by high performance liquid chromatograph-evaporative light scattering detector

为建立高效液相色谱法同时检测饮料食品中4种高倍甜味剂的方法,样品经水溶后超声波振荡提取,Inertsil C18色谱柱（250mm×4.6mm;5μm）分离,甲醇∶水（43∶57）（v/v）流动相洗脱,流速：1mL/m in;柱温：25℃;蒸发光散射检测器检测器条件：漂移管温度：85℃;气体压力：25psi;雾化器温度：25.2℃。进样量20μL。结果表明：安赛蜜、甜蜜素、三氯蔗糖和阿斯巴甜进样量0.1-10mg/mL的范围线性良好,相关系数分别为相关系数均为0.9947、0.9993、0.9998、0.9976。检出限分别为8.8μg/mL、4.0μg/mL、3.2μg/mL、1.4μg/mL：样品平均回收率在98.30%-99.00%之间,RSD%〈1.63%。本方法可同时对三氯蔗糖、甜蜜素、安塞蜜和阿斯巴甜等四种高倍甜味剂的分离与检测,该方法灵敏度高,重复性好,为快速检测食品中的高倍甜味剂提供了技术支持。

A quantitative assay for the determination of high sweetener,such as Acesulfame-K,Molasses,Sucralose and Aspartame in soft drinks was established by high performance liquid chromatography（HPLC）.High sweetener was extracted by Ultrasonic extraction.The separation was performed in the Inertsil C18（250mm×4.6mm i.d.5m）column,methanol：water（43 ∶57）（v/v）was used as mobile phase.Evaporative Light Scattering detector conditions were：drift tube temperature：85℃;gas pressure：25psi;atomizer temperature：25.2℃,sample volume 20μL.The result showed that the sweetener（Acesulfame-K,Molasses,Sucralose,Aspartame）had distinct linearity relationship with the concentration in the range of 0.1-10μg,the correlation coefficient was 0.9947-0.9998,the detection limit was 8.8μg/mL,4.0μg/mL,3.2μg/mL,1.4μg/mL respectively,RSD was 1.28%-1.63%.In conclusion,the method was accuracy,sensitive and feasible in the analysis of the residues of Sucralose,Molasses,Acesulfame-K and Aspartame in foods.