5'-核苷酸酶在白血病中的分布特点

Characteristic distribution of 5' Nucleotides in leukemia subtypes

目的:研究5'-核苷酸酶(5'-NT)在不同类型白血病、骨髓增生异常综合征(MDS)、非白血病患者和正常人中的分布特点。方法:分离100例不同白血病、3例MDS患者、9例非白血病患者和6例正常人骨髓有核细胞,以5'-单磷酸胞嘧啶核苷(5'-CMP)为底物进行细胞组织化学反应,电镜半定量分析5'-NT阳性细胞率和阳性指数。结果:5'-NT阳性反应产物主要位于有核细胞胞膜。急性髓系白血病(AML)M1、M2a、t(8;21)、t(15;17)各组细胞阳性率和阳性指数依次降低,阳性率组间差异有统计学意义(P<0.01),阳性指数差异无统计学意义;M1、M2a、t(8;21)、t(15;17)和M5患者5'-NT阳性细胞率和阳性指数均显著高于MDS、非白血病患者和正常人,差异有统计学意义(P<0.01);急性B淋巴细胞白血病(B-ALL)患者5'-NT阳性细胞率和阳性指数高于慢性淋巴细胞白血病(CLL),差异有统计学意义(P<0.05);CLL、MDS、非白血病患者和正常人的5'-NT阳性细胞率和阳性指数低,组间及两两比较差异无统计学意义。结论:不同白血病患者胞膜5'-NT表达与白血病细胞分化程度负相关。

Objective：To study distribution of 5＇nucleotides（5＇-NT） in patients with various leukemia,MDS,non-leukemic diseases,and healthy adults.Method：The nucleated cells were isolated from bone marrow of 100 patients with various leukemia subtypes,3 of MDS,9 of non-leukemic patients and 6 healthy donors.The reactivity of 5＇-NT was demonstrated by cytochemistry of 5＇-cytidine monophosphate（5＇-CMP） .The positive cell ratio and their index were measured with electron microscope on the base of ultrastructural observation semiquantitatively. Result：The positive product was located on plasma membrane.Positive ratios and indexes of M1,M2a,t（8;21） and t（15;17） were decreased in orderly.Their positive ratios were different statistically（P0.01） ,but positive indexes not.The positive ratios and indexes of all AML subtypes were higher than MDS,non-leukemia and healthy donors statistically（P0.05） .The positive ratio and index of B-ALL were higher than CLL statistically（P0.05） .The positive ratios and indexes of CLL,MDS,non-leukemic patients and healthy donors were lower and no significant difference between them.Conclusion：The results suggested 5＇-NT expression was an important index of blood cells,correlated with leukemic cell differentiation negatively.