摘要
目的:建立一套以相对长度为单位的测量人类单个染色体端粒长度的标准,测定正常人特定染色体长臂和短臂末端上的端粒长度。方法:采用荧光原位杂交定量法(Q-FISH)测定所有单个染色体端粒的信号强度,并转化为相对端粒长度(relative telomere length,RTL)进行比较。结果:对20例正常人外周血白细胞每条染色体上RTL值的测定显示,不同染色体臂上的端粒长度呈非随机分布并差异有统计学意义(F=8.923,P<0.01),其中2q、16p、17p、17q、19p、20q、22q、Yq的端粒最短(P<0.05),而3p、5p、Xp和Yp的端粒最长(P<0.05)。结论:用Q-FISH测定单个染色体RTL的方法易标准化,便于推广;正常人外周血白细胞中46条不同染色体上的端粒长度呈现特定的分布模式。
Objective:To established a relative telomere length(RTL) -based common standard for the measurement of single telomere lengths in human chromosomes,investigate the length distribution and variation among chromosome-specific telomeres in 20 normal people.Method:Using quantitative fluorescence in situ hybridization(Q-FISH) ,the fluorescence intensity of each single telomere was detected,then converted to an RTL,thus the RTL can be compared.Result:Telomere lengths at different chromosome end showed a nonrandom distribution statistically(F=8.923,P0.01) .The RTL at chromosome ends of 2q,16p,17p,17q,19p,20q,22q,Yq were shorter than others(P0.05) ,and the RTL at 3p,5p,Xp and Yp(p:chromosome short arm,q:chromosome long arm) were longer(P0.05) .Conclusion:It is easy to generate RTL using fluorescence intensity of the QFISH to determine chromosome-specific telomere length,which can be standardized and analyzed.There was specific distribution on chromosomes telomere length.
出处
《临床血液学杂志》
CAS
2010年第6期667-670,共4页
Journal of Clinical Hematology