CRSsNP中肥大细胞及其与嗜酸粒细胞炎症的关系

Mast cell and its relation to eosinophilic inflammation in CRSsNP

目的:观察不伴鼻息肉性慢性鼻-鼻窦炎(CRSsNP)的病理学特点,探讨肥大细胞及活化肥大细胞在CRSsNP中的作用及其与嗜酸粒细胞炎症的关系。方法:采用苏木精-伊红染色对CRSsNP和对照下鼻甲组织学特点进行观察以及对组织总炎性细胞、单个核细胞、浆细胞及嗜酸粒细胞进行计数分析。利用甲苯胺蓝和类胰蛋白酶免疫组织化学法分别检测肥大细胞和活化肥大细胞在CRSsNP组和对照组中的浸润情况,并探讨其与组织嗜酸粒细胞炎症的相关性。结果:CRSsNP组织中总炎性细胞、单个核细胞以及浆细胞数均高于对照组,而嗜酸粒细胞和黏膜固有层腺体数量于二者无明显差异。肥大细胞和活化肥大细胞计数呈相关性,但二者在CRSsNP组和对照组中均未见明显差异,且二者与组织嗜酸粒细胞炎症均无相关性。结论:CRSsNP较正常对照具有更重的炎症反应,但肥大细胞及其活化以及组织嗜酸粒细胞二者无差异,且肥大细胞及其活化与嗜酸粒细胞计数无相关性,提示肥大细胞及其介导的嗜酸粒细胞炎症在CRSsNP的发病中并不起重要作用。

Objective：To observe the pathologic characteristics,and investigate mast cell and its activation in chronic rhinosinusitis without nasal polyps（CRSsNP） and their relations with eosinophilic inflammation.Method：HE stain was used to observe tissue features and count total inflammatory cells,mononuclear cells,plasma cells and eosinophilils in lamina propria of CRSsNP and inferior turbinate.Toluidine blue stain and immunohistochemical stain for tryptase were used to detect mast cell and its activation respectively in CRSsNP and control,and their corelations with tissue eosinophilia were analysed.Result：CRSsNP has increased total inflammatory cells,mononuclear cells and plasma cells but comparable eosinophilils and lamina propria glands compared with inferior turbinate.Mast cells corelated with activated mast cells,but there was no difference between CRSsNP and control for both of them and there were no corelation between mast cell and its activation with tissue eosinophilia.Conclusion：CRSsNP has more serious inflammation but no more mast cell and its activation and eosinophil compared with inferior turbinate,and there were no corelations between mast cell and its activation with eosinophil count which suggests that mast cell and eosinophic inflammation mediated by it may not play an important role in the pathogenesis of CRSsNP.