整合素相连激酶和基质金属蛋白酶9在非小细胞肺癌中的表达及其与预后的关系

The Correlation of Integrin-linked Kinase and Matrix Metalloproteinases-9 Expression with Prognosis of Non-small Cell Lung Cancer

目的观察整合素相连激酶(ILK)和基质金属蛋白酶9(MMP-9)在人非小细胞肺癌(NSCLC)中的表达,初步探讨其与NSCLC预后的关系。方法选取2002年1月至2004年1月于南京医科大学附属第一医院手术切除的75例NSCLC患者标本,采用免疫组织化学方法检测NSCLC组织中ILK和MMP-9的表达水平,根据所得积分光密度(IOD)的中位数将75例患者分为ILK高表达组和低表达组,MMP-9高表达组和低表达组。采用Log-rank检验比较各临床病理特征及ILK、MMP-9表达对生存的影响,并对ILK和MMP-9的表达进行相关性分析。结果 ILK和MMP-9在NSCLC组织中均有表达,相关性分析显示:在本组75例患者中,ILK与MMP-9的表达呈正相关(r=0.79,P<0.05)。术后随访75例,随访至2009年1月31日时,中位生存时间30个月。ILK与MMP-9低表达组患者生存时间显著高于ILK和MMP-9高表达组(χ2=15.067,14.031,P<0.05);患者性别、年龄、吸烟史和病理类型对其生存期影响差异均无统计学意义(χ2=0.450,0.078,1.460,1.623,P>0.05);而肿瘤直径、淋巴结转移、TNM分期ILK及MMP-9表达对患者生存期影响差异均有统计学意义(2χ=3.936,15.169,20.529,15.067,14.301,P<0.05)。结论 ILK和MMP-9的表达水平影响NSCLC患者的预后,MMP-9可能通过ILK途径促进肿瘤细胞浸润、转移。因此,ILK和MMP-9的表达检测对评估NSCLC患者预后可能具有重要作用。

Objective To observe the expression of integrin-linked kinase（ILK） and matrix metalloproteinases-9（MMP-9） in human non-small cell lung cancer（NSCLC） and investigate the correlation of ILK and MMP-9 expression with the prognosis of NSCLC.Methods The expression of ILK and MMP-9 in 75 specimens of NSCLC resected from January 2002 to January 2004 were detected by immunohistochemistry.According to the median of integral optical density（IOD）,all patients were divided into the high or low ILK expression group and the high or low MMP-9 expression group.The relativity of ILK and MMP-9 was determined,and the relationship of survival time with clinical features including expression of ILK and MMP-9 was compared by Log-rank test.Results Both ILK and MMP-9 were expressed in NSCLC specimens.The expression between ILK and MMP-9 was positively correlated in 75 patients of our group（r=0.79,P0.05）.Patients with lower expression of ILK and MMP-9 had a significantly longer survival time than those with higher expression of ILK and MMP-9 in the postoperative follow-up（χ2=15.067,14.301,P0.05）.The survival time was not correlated with sex,age,smoking history or pathological type（χ2=0.450,0.078,1.460,1.623,P0.05）,while tumor diameter,lymph node metastasis,TNM stage,the expression of ILK and MMP-9 significantly influenced the survival time（χ2=3.963,15.169,20.529,15.067,14.301,P0.05）.Conclusion The expression of ILK and MMP-9 affects the prognosis of NSCLC.MMP-9 may advance infiltration and metastasis of tumor cells through ILK pathway.In summary,the expression of ILK and MMP-9 may play an important role in the evaluation of prognosis for patients with NSCLC.