胃癌彩色多普勒血流分级与微血管密度、血管内皮生长因子的表达及其相关性研究

Research on correlation of MVD,expression of VEGF and color Doppler flow imaging in patients with gastric carcinoma

目的探讨彩色多普勒超声检测胃癌血流分级与病理微血管密度(MVD)和血管内皮生长因子(VEGF)表达的临床病理关系。方法术前测定60例各期胃癌患者肿瘤区域和正常胃壁的彩色多普勒血流显像(CDFI),根据血流显示情况进行血流分级;术后免疫组化技术检测相对应标本内MVD、VEGF的表达。结果随着胃癌病灶区域内血流信号增多,血流分级增高,病理MVD计数和VEGF表达呈上升趋势(P=0.000);胃癌癌组织血流分级和MVD计数均高于癌旁正常组织(P<0.05),进展期高于早期(P<0.05);VEGF表达水平癌组织则低于癌旁正常组织(P=0.000),进展期高于早期(P=0.000);MVD值同VEGF表达密切相关(P<0.05),而胃癌癌旁正常组织进展期与早期血流分级、MVD值和VEGF表达比较差异均无统计学意义(P>0.05)。结论彩色多普勒超声所检测的胃癌内血流信号与病理MVD测值和VEGF表达有较好的相关性,胃癌术前CDFI血流检测可间接评价肿瘤组织内的血管生成状态,为胃癌的预后评估提供有益的信息。

Objective To explore the correlation between color Doppler flow imaging （CDFI） and microvessel density （ MVD ）, expression of vascular endothelial growth factor （ VEGF ） in patients with gastric carcinoma. Methods All 60 patients of all periods preoperative underwent CDFI for measuring the blood grades of gastric carcinoma and normal gastric mucosa tissues. Classified the blood grades according to the flow imaging. MVD and VEGF of corresponding samples were immunohistologically stained and measured after operations. Results MVD,VEGF demonstrated ascending tendency with the number increasing of flow imaging and the higher blood grades of gastric carcinoma tissues （ P = 0. 000 ）. The blood grades and MVD value in gastric carcinoma tissues was significantly higher than that in normal （ P 〈 0. 05 ） and higher in advanced than that in early stage（P 〈0. 05 ）. The expression of VEGF in advanced gastric carcinoma tissues was higher than that in early stage （ P = 0. 000） but lower than in normal ones （ P = 0. 000 ）. The MVD value in gastric carcinoma was significantly related with the expression of VEGF （ P 〈 0. 05 ）. The blood grades, MVD and the expression of VEGF were not significant different in normal tissues （P 〉 0. 05 ）. Conclusions Combination of CDFI and immunohistochemical parameters is useful for evaluating the angiogenesis of gastric carcinoma from different point of view.