益气活血法对AA大鼠滑膜细胞凋亡及血清细胞因子的影响

The influence of the therapy of Tonifying Qi and Eliminating Blood Stasis on Synovial Cell Apoptosis and Serum Cytokines in Adjuvant Arthritis Rats

目的选用黄芪注射液与川芎嗪注射液配伍,观察"益气活血法"对佐剂性关节炎(AA)大鼠滑膜细胞凋亡及血清细胞因子的影响。方法建立AA大鼠模型,设正常对照组、模型对照组、黄芪组、川芎嗪组、黄芪与川芎嗪配伍组和甲氨喋呤组进行对比观察,用药3周后检测各组大鼠足跖肿胀程度,滑膜细胞凋亡及血清细胞因子浓度。结果黄芪与川芎嗪配伍组能显著抑制注射足跖肿胀程度(P<0.05),显著提高滑膜细胞的凋亡率(P<0.01),并降低大鼠血清中IL-1、TNF-α、IL-6的水平(均P<0.01),上调IL-4的浓度(P<0.01),与甲氨喋呤组效果相当,甚至优于甲氨喋呤组。结论益气活血法可能通过促进AA大鼠滑膜细胞的凋亡,抑制某些炎性因子的释放,起到治疗类风湿性关节炎(RA)的作用。

Objective To observe the effect of Astragalus root and chuanxiongzine injection used in combination（called combination treatment group） on synovial cell apoptosis and serum cytokines in Adjuvant Arthritis（AA） rats to explore the mechanism of the therapy of tonifying qi and eliminating blood stasis in treating AA.Methods AA rat model was established.The rats were randomly divided into six groups：normal,model,Astragalus root treatment,chuanxiongzine treatment,combination treatment and MTX treatment.The influences of the therapy to the swelling of vola pedis,synovial cell apoptosis ratio and density of serum cytokines were observed.Results Combination treatment group distinctly attenuated the swelling of vola pedis,elevated synovial cell apoptosis ratio and decreased the contents of serum IL-1,TNF-αand IL-6,the content of IL-4 in serum increased,and the immune inflammatory response was inhibited,effects of which were similar and even superior to MTX.Conclusion The mechanism of the therapy of tonifying qi and eliminating blood stasis in treating AA may be restraining inflammation cytokines and decreasing the synovial cell apoptosis of AA rats,which reflects the advantages of Chinese medicine prescription under the direction of the treatment principles and methods of TCM.