葱白提取物对非酒精性脂肪肝大鼠COX-Ⅱ表达的影响

Effect of welsh-onion stalk extract on the expression of COX-Ⅱ protein in rats of nonalcoholic fatty liver

目的观察葱白提取物对非酒精性脂肪肝(NAFLD)大鼠COX-Ⅱ表达的影响。方法采用脂肪乳灌胃法复制非酒精性脂肪肝大鼠模型,同时施加不同剂量的葱白提取物进行治疗,并与易善复胶囊、绞股蓝总甙片做对照,观察不同处理组大鼠肝脏病理形态学的变化,应用免疫组化法观察大鼠肝内COX-Ⅱ的表达情况。结果与正常对照组相比,模型组大鼠肝组织可见重度脂肪变,炎症细胞浸润,肝细胞坏死,汇管区渗出,大鼠肝内COX-Ⅱ表达明显增高。与模型组相比,鲜葱白提取物低、中、高剂量组能显著减轻肝脏脂肪变性(P<0.01),COX-Ⅱ表达明显下降(P<0.01)。结论鲜葱白提取物可以减轻灌胃脂肪乳诱导的NAFLD大鼠COX-Ⅱ的表达,从而减轻NAFLD的炎症。

Objective To explore the effect of welsh-onion stalk extract on the expression of COX-Ⅱ protein in rats of nonalcoholic fatty liver disease（NAFLD）.Method The model of rats of nonalcoholic fatty liver disease（NAFLD）induced by fat emulsion by intragastric administration was used,which were treated with different dosages of welsh-onion stalk extract,compared with the groups administrated by Yishanfu Capsule and Gynostemma Pentaphyllum glycosides Tablet.The changes of pathology and morphology of the rats in the different experimental groups were observed,and the expression of COX-Ⅱ protein in rats of nonalcoholic fatty liver disease was also observed by using immunohisto-chemical method.Results Compared with the normal control groups,the rat liver tissues in the model group showed severe fatty change,inflammatory cells infiltration,hepatocyte necrosis,portal area exudation.The COX-Ⅱ in the livers were obviously increased.Compared with the model group,the liver fat denaturalization was obviously alleviated in the groups administrated different dosages of fresh fistular onion stalker cxtract（P0.01）,and COX-Ⅱwere also obviously decreased（P0.01）.Conclusion The fresh fistular onion stalker cxtract can reduce the expression of COX-Ⅱ protein in rats of NAFLD induced by fat emulsion by intragastric administration,therefore alleviate the inflammation of NAFLD.