异氟醚二次给药对体外人精子运动的影响

Dual effects of isoflurane on human sperm motility in vitro

目的研究异氟醚二次与单次给药对体外人精子运动的影响,以及异氟醚二次给药中首次与二次给药对精子运动增高幅度的影响。方法选取10例活力正常的人精液标本,给予1.4%、2.8%、5.6%异氟醚单次(或间隔1h二次)作用精液2h(或0.5h)、3h(或1h)、4h(或1.5h),采用计算机辅助精液分析系统观察异氟醚给药后的精子运动变化情况。结果 (1)1.4%～5.6%异氟醚二次作用精子0.5h、1h和1.5h时,其活动率和活力均显著高于对照组(P<0.05);1.4%～5.6%异氟醚单次作用精子2h、3h和4h时,其活动率和活力均显著高于对照组(P<0.05);(2)异氟醚二次作用精子0.5h、1h和1.5h时,5.6%异氟醚作用的精子活动率和活力显著高于1.4%和2.8%异氟醚作用的精子(P<0.05);异氟醚单次作用精子2h、3h和4h时,5.6%异氟醚作用的精子活动率和活力显著高于1.4%和2.8%异氟醚作用的精子(P<0.05);(3)1.4%～5.6%异氟醚二次作用精子0.5h、1h和1.5h时,其活动率和活力在同浓度异氟醚下依次显著高于单次作用2h、3h、4h时的精子活动率和活力(P<0.01);(4)5.6%异氟醚二次作用30min的精子,其提高精子活动率和活力的程度比首次作用更为明显(P<0.01)。结论异氟醚二次应用提高精子活力的作用不仅取决于浓度,而且与给药次数有关,二次异氟醚给药比单次给药更有效增强精子运动,异氟醚第一次给药对精子运动能力的增强可能具有"预处理"作用。

Objective To investigate whether two exposures to isoflurane would be more beneficial for improving sperm motility than would a single exposure and whether the amplitude in motility and viability of sperm was markedly increased by dual exposure compared with first exposure during two exposures. Methods Normal semen samples were obtained from 10 healthy men by masturbation. The single and dual effects at the clinical concentration （ 1. 4% -5. 6% ） on human sperm motility were observed by computer-assisted sperm analysis （ CASA） after isoflurane exposure. Results （ 1） Compared with control,dual exposures to 1. 4% -5. 6% isoflurane obviously increased sperm motility and viability for 0. 5 h,1 h and 1. 5 h,and sperm treated with 1. 4% -5. 6% isoflurane once showed an significant increase in the motility and viability of sperm for 2 h,3 h and 4 h as compared with control（ P 〈0. 05） ; （ 2） Dual exposures to 5. 6% isoflurane obviously increased sperm motility and viability for 30 min,60 min and 90 min,and sperm treated with 5. 6% isoflurane once showed an significant increase in the motility and viability of sperm for 2 h,3 h and 4 h as compared with 1. 4% or 2. 8% isoflurane（ P 〈0. 05） ; （ 3） Sperm motility and viability was increased by dual exposure to 1. 4% -5. 6% isoflurane for 0. 5 h,1 h and 1. 5 h as compared with single exposure to isoflurane at same concentration in turn for 2 h,3 h and 4 h（ P 〈0. 01） ; （ 4） The amplitude in motility and viability of sperm was markedly increased by dual exposure compared with first exposure during dual exposure to 5. 6% isoflurane for 0. 5 h（ P 〈0. 01）. Conclusions The effect of isoflurane on sperm motility depends not only on the concentration but also on the frequency of isoflurane exposure. Dualapplication of isoflurane seems to be more effective than single exposure in improving sperm motility at clinically relevant concentrations. It is likely that the first isoflurane exposure has a positive effect of preconditioning on the dual exposures to sperm.