金黄色葡萄球菌耐消毒剂基因及5类抗生素耐药相关基因检测

Drug-resistant gene and disinfectant-resistant gene in Staphylococcus aureus

目的了解本地区临床分离的金黄色葡萄球菌(SA)的耐药特点,探讨SA中耐消毒剂基因(qacA)、β-内酰胺类、氨基糖苷类、大环内酯-林可霉素-链阳菌素B类(MLSB)、四环素类、糖肽类药物耐药基因的存在情况。方法采用聚合酶链反应(PCR)法对20株SA进行β-内酰胺酶基因、氨基糖苷类修饰酶基因、MLSB类基因、四环素类基因、糖肽类基因、耐消毒剂基因检测。结果 PCR结果显示20株SA中13种耐药相关基因检出率分别为mecA75%、TEM90%、aac(6')/aph(2″)70%、ant(4',4″)15%、ant(6)-Ⅰ25%、ermA100%、ermC55%、msrA20%、msrB55%、tetM65%、qacA60%,vanA、vanB基因均为阴性。结论多数SA菌株具有耐多药特征,存在耐β-内酰胺类、氨基糖苷类、MLSB类、四环素类等多种抗生素耐药基因,且对胺类、胍类消毒剂耐受。

Objective To study the drug resistance genes and disinfectant-resistant genes of clinical Staphylococcus aureus isolates. Methods Common drug-resistant genes associated with beta-lactamases,aminoglycosides,MLSB,tetracyclines,sugar peptides and disinfectants were detected by PCR. Results The positive rates of 13 drug resistant genes in 20 SA strains were that,mecA 75% ,TEM 90% ,aac（ 6＇） /aph（ 2″） 70% ,ant（ 4＇,4″） 15% ,ant（ 6） -Ⅰ25% ,ermA 100% ,ermC 55% ,msrA 20% ,msrB 55% ,tetM 65% ,qacA 60% ,respectively. And the vanA,vanB were negative. Conclusions There are resistance genes to β-lactams,aminoglycosides,MLSB class,tetracycline and disinfectants in most of the SA strains.