高效液相色谱法测定废水中的全氟辛酸含量

Using HPLC to Determine the Purity of PFOA in the Waste Water

研究了高效液相色谱法检测工业废水中全氟辛酸含量的分析方法。采用kromasil(250 mm×4.6 mm×5μm)ODS C-18色谱柱,以乙腈与水的体积比50:50、加高氯酸(HClO4,质量分数1%)、三乙胺调pH=3.0为流动相,在该条件下可使试样中各组分完全分离,全氟辛酸的质量浓度在0.05～5 mg/L内线性良好(R=0.999 7、n=7、相对标准偏差1.60%),回收率在95.92%～105.2%。方法准确、快速、简便,可用于工业废水中全氟辛酸的检测。

An analytic method with HPLC is found to test perfluorooctanoic content in industrial wastewater with kromasil (250 mm×4.6 mm×5 μm) ODS C-18 chromatography column and mobile phase of methanol-acetonitrile (50:50/v pH=3 ) adding triethylamine toadjust pH, In this condition ,the components of perfluorooctanoic can be completely separated and a good linear relationship will beachieved to 0.05～5 mg/L(R=0.9997、n=7、RSD=1.60%) ,Recovery is about 95.92%～105.2%. The method was simple, rapid, accurate,which can be used to triethylamine detection in industrial wastewater.