应用HPLC技术分析盐藻β-胡萝卜素分子顺反异构体

Researching the method of HPLC for analysing cis trans isomers of β carotene in Dunaliella saline

采用ＨＰＬＣ技术对盐藻中β－胡萝卜素顺反异构体进行分分析，探索适宜的分析条件．所用仪器为ＷａｔｅｒｓＨＰＬＣ分析系统，检测波长λ＝４３６ｎｍ；灵敏度ＡｕＦｓ０．０５；色谱柱为：２５０×４．６Ｃ１８柱（Ｎｕｃｌｅ－ｏｓｉ７μｍ）；洗脱条件为：甲醇∶氯仿＝６０∶４０（Ｖ／Ｖ），流速为１ｍＬ／ｍｉｎ．所有操作过程均在避免高温、高湿度、强光照射、低ｐＨ值条件下进行．

Carotenoids have big family in which β carotene have mang different stereoisomers.Our experiment using high performance liquid chromatography to analyse the cis trans isomers of Dunaliella salina β carotene,in order to get the optimun analylical conditions.We use WATERS HPLC system to get suitable conditions:1.Chromatographic columns:C 18 (250×4.6,Nucleosi 7μm);Mobile phase:Methanol∶Trichloromethane=60∶40( V/V );Flow rate:1 mL/min;detection,λ=436nm;temperature at 0～10℃.2.Two kinds of cis isomers and trans isomers are found.3.All of the experiments are in the conditions avoiding high temperature,stong,light,low pH.