转基因与非转基因杂种山杨组培苗内源激素的比较

A Comparative on the Plant Endogenous Hormones of Transgenic and Non-Transgenic Hybrid Aspen Tissue-Cultured Plantlets

以转基因杂种山杨无性系为材料,非转基因无性系为对照,采用间接酶联免疫吸附测定法(ELISA),测定不同基因型和不同生根类型组培苗的植物内源激素(IAA,ABA和GA)含量的变化,探究植物内源激素含量及激素间相互作用对转基因杂种山杨无性系组培苗生根的影响。结果表明:不论是转基因还是非转基因山杨无性系不同生根类型间在IAA和ABA含量上均达到显著和极显著差异;不同生根类型的杂种山杨,赤霉素GA的含量始终处于高值水平,比较稳定,保持在400～500ng·g-1左右。转基因组培苗内源脱落酸ABA的含量较低,在150ng·g-1左右时,促进生根。内源生长素IAA含量在200ng·g-1以下时,有利于组培苗的生根。非转基因植株在组培过程中均可生根,但分为直接生根和愈伤组织生根2种类型,直接生根类型的3种激素含量均高于愈伤组织生根类型。低的IAA/ABA,高的GA/ABA和GA/IAA比值是植株生根的重要因素。

In the present study,one clone of transgenic hybrid aspen（Populus tremula × P.tremuloides） from tissue culture was used as the materials and non-transgenic clone as the control.The contents of endogenous plant hormones,including IAA,ABA and GA,were measured by ELISA in different genotypes and different rooting types.The purpose was to study effects of changes in endogenous plant hormones,and their interaction on rooting of hybrid aspen during tissue culture.The results showed that there was extremely significant variation in IAA and ABA contents among different rooting types of the ramets,regardless transgenic or non-transgenic plants.GA content from different rooting ramets was all highest with about 400-500 ng·g-1.In transgenic ramets,ABA and IAA concentrations were relatively low in about 150 and 200 ng·g-1,respectively,at which they were good for promoting the rooting of the plants.Non-transgenic plants in different rooting types all could get the roots,but the phytohormone contents in direct rooting ramets were generally higher than those of the callus rooting ramets.The low IAA /ABA and high GA /ABA and GA /IAA played an important role in the plant rooting.