文冠果悬浮细胞系的建立及细胞生长特性

Establishment of Suspension Cell Cultures and Cell Growth Characteristics of Xanthoceras sorbifolia

以文冠果成熟种胚诱导的愈伤组织为材料,进行悬浮培养细胞系的建立及细胞生长特性的研究。结果表明:文冠果细胞悬浮培养的初始接种量在100mL三角瓶40mL液体培养基中以2.0g为宜,最适激素浓度以2,4-D,6-BA,NAA均为0.5mg·L-1为宜,细胞活力在接种第3天时达到最大,细胞分裂指数在接种第10天时达到最大,鲜质量增长量在第14天时达到最大。

Suspension-cultured cell lines of Xanthoceras Sorbifolia were established by using callus induced from mature embryos.Growth characteristics of the cell cultures were further studied.The results showed that the optimum initial amount of inoculum amount was 2 g in 40 mL liquid culture medium,and the optimum concentration of plant hormones was 0.5 mg·L-1 2,4-D and 0.5 mg·L-1 6-BA and 0.5 mg·L-1 NAA,respectively.The highest cell mitotic index appeared on day 10,the cell activity was highest on day 3,and the fresh weight peaked on day 14.