大鼠复发性结肠炎模型的建立

Establishing a recurrent colitis model in rats

目的建立大鼠复发性结肠炎模型。方法雄性Wistar大鼠随机分为正常对照组、急性结肠炎组、自发性愈合组和复发性结肠炎组,用80mg/kg 2,4,6-三硝基苯磺酸(TNBS)50%乙醇溶液建立大鼠溃疡性结肠炎(UC)模型,在造模第16天时,用30mg/kgTNBS 50%乙醇溶液进行第2次致炎诱导复发性结肠炎,对模型进行大体和组织学观察评分,并检测病变组织中的髓过氧化物酶(MPO)活性。结果与正常对照组、急性结肠炎组和自发性愈合组比较,复发性结肠炎组大鼠大体和组织学损伤评分均明显增高(P<0.05或P<0.01);与正常对照组和急性结肠炎组比较,复发性结肠炎组大鼠结肠组织中MPO活力明显升高(P<0.05或P<0.01)。结论采用TNBS/乙醇在首次致炎两周后进行第2次致炎,可以模拟UC缓解-复发交替出现的特点,是较理想的人类UC模型,可以应用于UC缓解期复发的研究。

Objective To establish a recurrent colitis model in rats.Methods Wistar male rats were randomized divided into four groups：normal control group,acute colitis group,spontaneous healing group and recurrent colitis group.Recurrent colitis was induced in rats by rectal administration of 80 mg/kg 2,4,6-trinitrobenzenesulfonic acid solution（TNBS） dissolved in 50%ethanol, and then the rats were instilled again with 30 mg/kg TNBS into the colon on the 16th day after the first induction ulcerative colitis.All the rats were sacrificed 7 days after second induced ulcerative colitis by TNBS.Macroscopical and microscopical damage scores and myeloperoxidase（MPO） activity were assessed,and the colonic injury and inflammation were evaluated respectively.Results Compared with normal control group,acute colitis group and spontaneous healing group, macroscopical and microscopical damage scores and MPO activity were notably increased in the recurrent colitis group.Conclusion A recurrent colitis model in rats has been established.This twice-induced animal colitis by TNBS is more similar to human inflammatory colitis which can be used in the study of the remission-period recurrence.