A型肉毒类毒素聚乳酸微球制备及酶联免疫吸附测定方法

Preparative conditions for polylactide microspheres containing botulinum A-type toxoid and the establishment of detection

目的以A型肉毒类毒素为模型药,探讨聚乳酸用于包裹生物大分子的可能性。方法采用水-油-水复乳溶剂挥发法制备微球,并测定粒径和包封率,用间接酶联免疫吸附测定法检测免疫鼠血清中的抗体水平。结果获得了60%以上的包封率,微球粒径可控制在30～200μm,微球在体内缓慢释放诱导抗体产生。结论采用水-油-水复乳溶剂挥发法制备微球,成功地制备了A型肉毒类毒素免疫微球,可用于运载生物大分子。

Objective To explore the possibility of entrapping biopharmaceutical macromolecular by preparing polylactide microspheres containing botulinum A-type toxoid as model drug. Methods The vaccine was entrapped by employing a water-in-oil-in-water emulsion solvent evaporation technique.The effects of process parameters on particle size and protein entrapment were investigated.The dynamic antibody level in serum was monitored by indirect enzyme-link immunosorbent assay to evaluate the slow-release.Results It was found that microspheres with loading efficiency up to 60%could be produced and 30-200μm in diameter could be controlled. The microspheres induced better antibody response and had slow-release effect.Conclusion The preparation of polylactide microspheres with an entrapped vaccine botulinum A-type toxoid is studied.The polylactide microspheres made by water-in-oil-in-water emulsion solvent evaporation technique can be used as carriers for delivering biopharmaceutical macrornolecular.