摘要
目的:利用超高效液相-质谱法建立同时测定降脂颗粒中6种活性成分含量的方法。方法:色谱柱为Acquity UPLC BEH C18柱(2.1mm×50mm,1.7μm),流动相为甲醇-0.1%甲酸水梯度洗脱,选择离子监测。结果:在线性范围内相关系数良好,均大于0.9992,日内、日间精密度良好,RSD值均小于3%,加样回收率在97.58%-103.12%,符合要求。结论:本方法快速灵敏,测定结果准确可靠,可用于降脂颗粒的质量控制。
AIM： A novel and rapid method using an ultra-performance liquid chromatography coupled with mass spectrometry was developed and validated for the simultaneous determination of six constituents in ‘Jiang-Zhi’ granule. METHODS： An acquity UPLC BEH C18 column （2.1 mm × 50 mm, 1.7 μm） was utilized. Methanol and 0.1% formic acid were adopted in the elution gradient. The selective ion monitor （SIM） mode was used to detect the target compounds. RESULTS： The established method showed a good linearity （R2 0.999 2） over the investigated concentration ranges, good inter-day and intra-day precisions （less than 3%） and good recoveries （from 97.58% to 103.12%） for all six target compounds. CONCLUSION： The contents of six main components in ‘Jiang-Zhi’ granule could be determined using the established method.
出处
《中国天然药物》
SCIE
CAS
CSCD
北大核心
2010年第6期449-455,共7页
基金
supported by the National Nature Science Foundation of China (No 30873260)
the Talents Scheme from the Science and Technology Commission of Shanghai Municipality (No 09XD1403800)
the China Postdoctoral Science Foundation (No 20090460645)
the Supporting Project for Elitists in the New Century from The Ministry of Education (No NCET-07563)
the Shanghai Leading Academic Discipline Project (Nos J50305 and E3008)~~
关键词
超高效液相-质谱法
中药
降脂颗粒
Ultra performance liquid chromatography–mass spectrometry
Traditional Chinese medicine
‘Jiang-Zhi’ granule
