摘要
应用荧光光谱研究了2,6-二(邻甲基苯亚甲基)环己酮(用A表示)与牛血清白蛋白(BSA)间的结合作用,确定了A对BSA的荧光猝灭过程的猝灭机理.测定了不同温度下该结合反应的结合常数,结合位点数,热力学参数.结果表明A与BSA以1∶1的比例结合形成复合物,结合过程主要是熵驱动,相互作用主要为疏水作用力.
The interaction of 2,6 - bis（2 - methylbenzylidene）cyclohexan - 1 - one（A）and bovine serum albumin（BSA） was studied by fluorescence spectrum. It ＇s proved that the fluorescence quenching of BSA by A is a result of A - BSA complex. The binding constant, the number of binding sites and thermodynamic parameters were measured at different temperatures by fluo- rescence quenching method. Experimental results showed that the quenching mechanism is dynamic quenching procedure, the complication substance was produced through the A binding with BSA as 1＂1.
出处
《凯里学院学报》
2010年第6期77-79,共3页
Journal of Kaili University
关键词
2
6-二(邻甲基苯亚甲基)环己酮
牛血清白蛋白
荧光光谱
2,6 - bis（2 - methylbenzylidene）cyclohexan - 1 - one
bovine serum albumin
fluo-rescence spectrum
