卵母细胞体外培养时间对单精子胞浆内注射临床结局的影响

Impact of oocyte preincubation time on clinical outcomes after intracytoplasmic sperm injection

目的探索最适合的单精子胞浆内注射(ICSI)时机。方法根据从患者取卵结束到开始ICSI的时间(t),将患者分为3组:A组(2h≤t<4h,n=60),B组(4h≤t≤6h,n=85),C组(6h<t≤8h,n=62)。回顾性分析了卵母细胞体外培养时间对于ICSI患者的受精、胚胎发育及临床结局的影响。结果 B组及C组患者的临床妊娠率及植入率均显著高于A组。结论适度的卵母细胞体外培养时间(4～8h)有利于改善ICSI患者的临床结局。

【Objective】To determine the optimal timing of intracytoplasmic sperm injection （ICSI）.【Methods】 According to the oocyte preincubation time （t） between oocyte retrieval and ICSI,patients were assigned into three groups： Group A （2 h≤t 4 h,n =60）,Group B （4 h≤t ≤6 h,n =85） and Group C （6 ht ≤8 h,n =62）.Impact of oocyte preincubation time on fertilization,embryo development and clinical outcomes after intracytoplasmic sperm injection was analyzed retrospectively.【Result】Clinical pregnancy rates and implantation rates in Group B and Group C were significantly higher than Group A.【Conclusion】Appropriate preincubation time could improve clinical outcomes after ICSI.