发育中小鼠肾淋巴管内皮细胞透明质酸受体-1^+细胞的研究

Research of LYVE-1^+ macrophage in developing mouse kidney

目的探讨淋巴管内皮细胞透明质酸受体-1(LYVE-1)阳性细胞在发育中小鼠肾内的分布及形态特点。方法胚胎期第13～16天(E13～16)的胎鼠和出生后第1、4、14和21天(P1～21)及成年的小鼠肾,震动切片后进行LYVE-1免疫组织化学和免疫荧光染色。结果 LYVE-1+细胞在小鼠胚胎期第13天的肾髓质开始表达,这种细胞的数量逐渐增多直到出生后第4天,然后逐渐减少,直到出生后第21天完全消失。这些细胞有粗而不规则的突起,出生后这些突起逐渐变细变长。免疫荧光证实其为巨噬细胞。结论 LYVE-1+细胞为巨噬细胞,并在淋巴管的发生中起一定的作用。

【Objective】 To investigate the distribution and morphological changes of LYVE-1＋ cells in developing mouse kidneys.【Methods】 The mouse kidneys from embryonic day 13 （E13） to E16,from neonates at pup day 1 （P1） to P21 and adults were vibratomed,then LYVE-1 immunostained and immunefluorescent stained.【Results】 LYVE-1＋ cells appeared firstly in the medulla at the E13 stage and the number increased gradually until P4,then reduced and disappeared completely at P21.These cells had several thick irregular shaped cytoplasmic processes,which decreased gradually in thickness but increased in length after birth.These cells were macrophage approved by immunofluorescence.【Conclusion】 LYVE-1＋ cells were macrophage and these cells may be associated with devolopment of intrarenal lymphatic vessels.