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二甲双胍通过激活腺苷酸活化蛋白激酶抑制大鼠心脏成纤维细胞增殖及胶原合成 被引量：2

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摘要 目的探讨降糖药物二甲双胍(metformin)对心脏成纤维细胞(CF)增殖及胶原合成的影响及可能的机制。方法培养新生SD大鼠CF,以血管紧张素Ⅱ(AngⅡ)刺激CF增殖,同时分别单独或联合应用二甲双胍及一磷酸腺苷活化蛋白激酶(P-AMPK)抑制剂Compound C,用MTT法测CF增殖,3H-脯氨酸掺入率法测定CF胶原合成,Western blot测定腺苷酸活化蛋白激酶(AMPK)及P-AMPK水平。结果 CF经AngⅡ刺激24h后,显著增殖,同时胶原率提高;二甲双胍显著抑制了AngⅡ诱导的CF增殖及胶原合成增加;Compound C完全抑制了二甲双胍的这一效应,而单独应用Com-pound C并未进一步加重AngⅡ诱导的CF增殖及胶原合成。二甲双胍显著提高AngⅡ刺激的CF的P-AMPK水平,同时Compound C抑制了二甲双胍对AMPK的磷酸化激活。结论二甲双胍显著抑制了AngⅡ诱导的CF增殖及胶原合成增加,这一效应可能由二甲双胍激活AMPK介导。
作者 张成喜 潘思年 彭朝权 熊肇军 吴震 余舒杰
机构地区 中山大学附属第三医院心内科 中山大学附属第三医院儿科
出处 《中华临床医师杂志（电子版）》 CAS 2010年第8期138-140,共3页 Chinese Journal of Clinicians(Electronic Edition)
关键词 成纤维细胞 二甲双胍 环AMP依赖性蛋白激酶类
分类号 R541.3 [医药卫生—心血管疾病]
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参考文献14

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同被引文献21

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中华临床医师杂志（电子版）
2010年 第8期

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