安徽黄精属五种植物的同工酶分析

A STUDY ON ISOZYMES OF 5 SPECIES IN POLYYONATUM MILL

本文采用聚丙烯酰胺凝胶电泳技术分析了安徽产黄精属（ｐｏｌｙｙｏｎａｔｕｍｍｉｌ．）五种七个居群植物的过氧化物酶（ＰＯＤ）、酯酶（ＥＳＴ）同工酶，比较了居群间酶谱差异，并对获得的酶谱资料进行了聚类分析（ＵＰＧＭＡ）．结果表明：１）七个居群植物可明显分为５个有效种：Ａ１和Ａ２为一种，Ｂ１和Ｂ２为一种，Ｃ，Ｄ，Ｅ分别为三个不同种，此结果与有关形态分类的研究结果一致；２）酶谱特征显示居群间呈多样性；且种间居群差异明显大于种内居群差异；３）本文认为Ｂ１，Ｂ２（长梗黄精）为较进化的种，Ｅ（琅琊黄精）为较古老种．

This paper presented the interspecific relationship among seven populations of five species of polyyonatum mill producted Anhui province based on the comparative study of two isozymes (POD. EST)using PAGE and analysed the date obtained from the zymogram by cluster analysis(UPGMA). The results were: 1)The seven populations can be classficated into five species, A1 and A2 、 B1 and B2、C 、D 、E respectively. 2)The characteristic of zymogram indicated diversity among populations, and the variations were bigger among populations of species then within population of species. 3) The results indicated that P.filipes mirr was more evolution and P.langyaense was older.