不同感染剂量MDRV对番鸭免疫反应和细胞毒性作用的影响

Influence of different infective dose of MDRV on immune responses and cytotoxic activity in muscovy ducks

用不同剂量番鸭呼肠孤病毒(MDRV MW9710株)感染8日龄番鸭后,通过检测血液中淋巴细胞对ConA、LPS的反应和NK细胞、细胞毒T细胞(CTL)的细胞毒性作用,探讨MDRV感染对番鸭免疫细胞功能和细胞毒性作用的影响。结果显示,不同感染剂量MDRV均会抑制番鸭血液淋巴细胞对ConA、LPS的增殖反应,降低NK细胞和CTL细胞的杀伤活性,且影响程度与剂量相关;同时感染番鸭生长缓慢,脾脏肿大,胸腺和法氏囊缩小。上述结果表明,MDRV感染能导致番鸭免疫抑制,且细胞免疫抑制程度与感染病毒量有关。

To access the effect of different infective dose of MDRV（muscovy duck reovirus） on the immune response in muscovy ducks,immune responses of the lymphocytes in the blood to ConA and LPS,cytotoxic activity of cytotoxic T lymphocytes（CTL） and natural killer cells were detected.The results showed that MDRV infection,not only at higher dose but at low dose,could cause the immunosuppression of lymphocytes proliferation response to ConA and LPS in muscovy ducks.The cytotoxic activities of NK cel1 and CTL were reduced in muscovy ducks infected with MDRV.And muscovy ducks infected with MDRV were slow-growth,splenomegaly,thymus and bursa atrophy.So MDRV infection appeared to depress certain immune response,that was related to the infection dose.