南非Ⅱ型口蹄疫病毒抗原表位的筛选及抗原性分析

Screening of major epitopes of foot-and-mouth disease virus type SATⅡ and their antigenicity analysis

目前,我国南非Ⅱ型（SATⅡ）口蹄疫病毒（FMDV）的防控形势十分严峻,为了防止SATⅡ型FMD的跨境传入,迫切需要建立其特异的检测方法。本研究以SATⅡ型FMDV结构蛋白氨基酸序列为依据,利用分子生物学软件分析了FMDV结构蛋白VP1~VP3上可能的抗原表位,并人工合成了8条表位多肽。通过采用SATⅡ型FM-DV阳性血清进行ELISA反应,检测其反应原性;通过采用与载体蛋白偶联的合成肽免疫小鼠,测定小鼠血清中抗体效价,检测合成肽的免疫原性。结果表明,合成的8条多肽均能与SATⅡ型FMDV阳性血清结合,其中的6条多肽免疫小鼠后能产生针对多肽的抗体。本研究为利用串联表位为抗原检测SATⅡ型FMDV抗体方法的建立奠定了基础。

In order to monitor and control the trans-boundary transmission of Southern-Africa type（SAT） Ⅱ of foot-and-mouth disease（FMD）,specific detection method should be established especially for FMDV type SATⅡ which had outbreak in Asia countries before.Based on the amino acid sequence analysis of VP1-VP3 region of FMDV type SATⅡgenome,eight peptides predicted as antigenic epitopes were mechanically synthesized and the antigenicity were analyzed.Results indicated that all of the 8 peptides could specifically reacted to antisera against SAT Ⅱ of FMDV,6 of them could induce specific antibody when BALB/c mice were immunized for 3 times.This study developed a good foundation for using peptide fusion protein as antigen in the detection of FMDV type SAT Ⅱ antibody.