奶牛临床型乳房炎Nisin抗菌肽治疗后日产奶量及主要乳成分的变化

Changes of daily milk production and major constituents of milk after intramammary infused with Nisin of bovine clinical mastitis

选取临床型乳房炎自然发病病例9头,乳房内灌注乳酸链球菌素(Nisin)进行治疗,通过检测日产奶量以及停药后2,7,14,21 d的主要乳成分指标,确定Nisin乳房灌注剂对临床型乳房炎病例泌乳性能的影响情况。结果显示,乳房内灌注Nisin后患病乳区乳腺组织得到一定程度的修复,日产奶量逐渐恢复,停药后8 d的日产奶量与发病前6 d相比,平均降幅2.5 kg。治愈后患病乳区牛奶乳脂肪、乳蛋白、乳糖和非脂乳固体含量都呈现上升趋势,但与同期相比,略低于非用药乳区混合牛奶;其中,乳糖含量增加幅度较快,除停药后2 d的治疗乳区外,乳糖含量均在4.7%以上,提示奶牛乳房炎病例经Nisin乳房灌注治疗后,受损乳腺组织修复较快,乳腺上皮细胞合成乳成分的能力大幅度提高。由此可见,Nisin对奶牛临床型乳房炎具有良好的治疗作用。

Nine dairy cows with clinical mastitis were selected and intramammary administrated 6 times with Nisin-based formulation.Daily milk production and major constituents of milk were analyzed to evaluate the effect of Nisin-based formulation treatment on lactating performance.Daily milk productions of all the mastitic cases gradually recovered,but the milk production at 8 d after the end of treatment decreased by an average of 2.5 kg compared with 6 d before infection.Milk fat,protein,lactose and solids-non-fat contents of milk collected from treated quarters increased after intramammary infusion of Nisin-based formulation,but they were lower than those from untreated quarters（composite milk samples）.The concentration of milk lactose content was above 4.7% in all treated quarters except for 2 d after intramammary infusion,suggesting that mammary tissues damage may recover quickly and the synthesis of epithelia cells increase in large extent.In conclusion,Nisin-based formulation showed efficacy in the treatment of bovine clinical mastitis.