新疆野生盘羊mtDNA D-loop序列的多态性

Mitochondrial DNA genetic diversity of wild argali

根据GenBank中3条羊亚科动物线粒体DNA全序列设计并合成1对引物,成功扩增了新疆天山亚种野生盘羊线粒体(mtDNA)控制区(D-loop)全长序列片段,并进行序列分析。结果显示,1号和2号盘羊mtDNA D-loop序列全长分别为1 070,1 169 bp;在检测的2个个体上共发现73个突变位点,其中有7个插入,50个转换,11个颠换,5个缺失,表明碱基的替换有明显偏倚;1号和2号新疆天山亚种野生盘羊mtDNA D-loop区核苷酸突变位点分别为35个和38个,核苷酸变异率分别为3.27%和3.25%。这一结果表明,新疆天山亚种野生盘羊群体线粒体D-loop区存在着丰富的多态性,同时进一步说明了我国新疆野生盘羊遗传资源比较丰富。

The mtDNA D-loop complete sequences of wild argali（O.a.karelini）,Xinjiang,China were specificitly amplified by PCR technology.The PCR product was purified by agarose.The mtDNA D-loop sequences were sequenced and analyzed.The result showed the length of mtDNA D-loop complete sequence was 1 070 bp and 1 169 bp,respectively,in No.1 and No.2 argali.There were 7 insertion loci,50 transitions loci,11 transversion loci and 5 deletions loci of 73 mutational loci which were found in two wild argalis and transitions was the major mutational type.The mutational loci of mtDNA D-loop sequence,from No.1 and No.2 argali（O.a.karelini）,were 35 and 38;mutational rate were 3.12% and 3.25%.It showed that the wild argali population has high variation in mtDNA D-loop sequence.Meanwhile,it further showed that there was plentiful genetic resource of wild argali Xinjiang,China.