热毒宁注射液中栀子提取浓缩液萃取工艺研究

Study on the Counter-current Extraction Process of Fructus Gardeniae

目的:考察热毒宁注射液中栀子提取浓缩液萃取工艺,比较常规搅拌萃取、连续逆流萃取两种不同萃取方法,优选最佳萃取方法。方法:以栀子苷转移率及固含物转移率为指标,确定最佳萃取方法,并采用正交试验优选最佳工艺条件。结果:从栀子苷转移率及固含物转移率分析,连续逆流萃取明显优于常规萃取,连续逆流萃取最佳工艺条件:药液温度20℃,萃取药液pH值为3,萃取溶剂用量为萃取药液的6倍,萃取溶剂速度为250 mL.min-1。结论:连续逆流萃取法萃取效率高、时间短,技术操作简便,能有效地提高生产效率。

Objective： To compare the different extraction methods of conventional agitated extraction and countercurrent extraction with Gardeniae extract concentrated liquid and optimize the extraction conditions.Methods： The orthogonal test was used to optimize the process conditions with the transfer rates of geniposide and solid material as the indexes.Results： From the analysis of the transfer rates,the countercurrent extraction method was superior to the conventional extraction method.The optimum conditions for countercurrent extraction were as follows： the extraction liquid temperature was 20℃ at pH 3,the extraction solvent was 6 times of the extraction liquid,the solvent extraction rate was 250 mL·min-1.Conclusion： The optimized method is simple and has high efficiency,it can effectively improve the productivity of Gardeniae extract concentrated liquid.