摘要
目的：建立快速测定人全血中西罗莫司浓度的高效液相色谱方法。方法：色谱柱为Zorbax Eclipse XDB C18（4.6mm×150mm,5μm）;流动相为甲醇-乙腈-去离子水（50∶22∶28,v/v）;流速1.2mL.min-1;紫外检测波长278nm;柱温50℃。结果：西罗莫司浓度测定的线性范围为2.5~50.0μg.L-1,最低检测浓度为2.0μg.L-1,平均回收率为103.2%,日内RSD小于5%,日间RSD小于4%。结论：本方法简便、快速,定量准确,可用于西罗莫司临床药动学研究,也可用于西罗莫司常规血药浓度监测。
Objective： To establish a rapid and feasible high-performance liquid chromatographic（HPLC） method for the determination of Sirolimus（SRL） in human plasma.Methods： Prior to analysis,SRL was isolated from plasma sample using ultra filtration.The extracts were injected onto an Zorbax Eclipse XDB C18（4.6 mm×150 mm,5 μm） column at 50℃ with detection UV wavelengths set at 278 nm.The mobile phase was consisted of Meth-ACN-H2O（50∶22∶28,v/v）,at a flow rate of 1.2 mL·min-1.Result： Calibration curves were linear over a concentration range of 2.5～50.0 μg·mL-1 for SRL.The average recoveries of SRL were 103.2%.The within-day precision and between-day precision of SRL were less than 5% and 4%.Conclusion： This method is simple,quick and accurate and can be used for routine monitoring and clinical study of SRL pharmacokinetics.
出处
《药学与临床研究》
2010年第6期580-582,共3页
Pharmaceutical and Clinical Research
