龙牙楤木多糖诱导人肝癌SMMC-7721细胞凋亡机制研究

AEPS induces apoptosis in SMMC-7721 hepatocellular cancer cells and its mechanism

目的:探讨龙牙楤木多糖(AEPS)对体外培养人肝癌SMMC-7721细胞凋亡的影响,并探讨其可能的作用机制。方法:荧光显微镜观察细胞形态学变化,流式细胞术(FCM)检测SMMC-7721细胞的凋亡率;Western blot检测不同浓度AEPS作用36小时凋亡蛋白survivin、caspase-3及bcl-2的表达情况。结果:与对照组比较,AEPS不同浓度剂量组Hoechst33258/PI荧光染色出现典型的凋亡形态学改变;FCM检测表明SMMC-7721细胞凋亡率各实验组均明显高于对照组(P<0.01),且呈时间剂量依赖性;Western blot显示与对照组相比,各实验组AEPS可显著下调SMMC-7721细胞survivin和bcl-2的表达(P<0.01),而caspase-3的表达显著增加(P<0.01)。结论:AEPS能明显诱导人肝癌SMMC-7721细胞凋亡,其机制可能与改变凋亡相关基因survivin、caspase-3及bcl-2的表达有关。

Objective：To study the mechanism of apoptosis induced by AEPS in SMMC-7721 hepatocellular cancer cells.Methods：The fluorescent microscope was utilized to observe the morphological changes of apoptosis,apoptosis rate were detected with flow cytometry.Western blot was utilized to detect the expression of survivin,caspase-3 and bcl-2.Results：Typical morphological changes of apoptosis in SMMC-7721 cancer cells could be observed by the fluorescence microscope.Compared with that of the control cells,apoptotic rate of the cells treated with AEPS was increased signifcantly（P0.01）and in a dose-and time-dependent manner.Western blot showed that survivin and bcl-2 expression was signifcantly decreased（P0.01）,whereas caspase-3 expression was signifcantly increased（P0.01）in SMMC-7721 cancer cells treated with AEPS.Conclusion：AEPS can markedly induce apoptosis in SMMC-7721 hepatocellular cancer cells.The mechanism might be related to the change of survivin,caspase-3 and bcl-2 expression.