摘要
目的观察基质金属蛋白酶-8(MMP-8在牙本质龋损中的表达,探讨MMP-8与龋病发展的关系,希望能为龋病的预防和治疗开辟新的思路。方法标本选自因龋未经治疗的第三磨牙,按临床及X线诊断分为牙本质浅龋、牙本质深龋各10颗,无龋坏第三磨牙10颗为对照组。采用免疫组织化学方法观察MMP-8在不同牙本质龋损中表达。结果正常组MMP-8在成牙本质细胞层略有着色;牙本质浅龋组成牙本质细胞增生活跃,前期牙本质增厚,染色阳性,牙髓细胞染色加深;牙本质深龋组成牙本质细胞呈阳性表达,牙本质基质中呈散在条索性表达,牙髓组织中成纤维细胞、血管内皮细胞强阳性表达,在修复性牙本质中MMP-8表达强阳性。结论 MMP-8参与龋病的进展过程。
Objective To investigating the relationship of and caries by observing the expression of MMP-8 in the dentine caries.Methods 30 third molars with untreated caries were extracted and divided into three groups as enamel caries,superficial caries of dentin and deep caries according to clinical examination and radiography examination,and every group include 10 cases as well as 10 cases of normal third molars teeth as control.The expression of MMP-8 was observed by the way of immunohistochemistry.Results MMP-8 in odontoblast was slightly stained in normal group and weakly positive in enamel caries group.In superficial caries of dentin in which odontoblasts proliferate actively and predentine became thicken,exhibited positive staining,and deepen stained in pulp cell.In the deep caries group,MMP-8 was positive in odontoblast and strong positive in fibroblast,vascular endothelial cells of pulp tissue and in reparative dentine.Conclusion MMMP-8 is involved in the progression of caries.
出处
《中国实验诊断学》
北大核心
2010年第12期1951-1953,共3页
Chinese Journal of Laboratory Diagnosis
基金
长春市科技局基金资助课题(合同编号2004064)
